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The cell line was established from skin taken from the breast of a female with macromastia.

The cell line was established from skin taken from the breast of a female with macromastia.

The cell line was established from skin taken from the breast of a female with macromastia.

female

Black

18 years

The cell line was established from skin taken from the breast of a female with macromastia.

Cells senesce after approximately 56 population doublings.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

culture medium 95%; DMSO, 5%

Temperature: 37.0°C