| 產(chǎn)品名稱 |
G-3-5 |
| 商品貨號(hào) |
B164481 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Autopsy |
| Applications |
It is useful for immunofluorescence, immunocytochemistry, and Western blotting procedures. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Animals were immunized human brain homogenate of multiple sclerosis plaques obtained at autopsy. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against alpha actinin |
| Cellular Products |
immunoglobulin; monoclonal antibody; against alpha actinin |
| Comments |
Animals were immunized human brain homogenate of multiple sclerosis plaques obtained at autopsy. Spleen cells were fused with NS-1 myeloma cells. The antibody recognizes a novel intermediate filament associated protein (MW 100000) with similarity to alpha actinin. This antibody intensely stains astroglia and related macroglia in cryostat sections of human and rat central nervous system tissue. It is useful for immunofluorescence, immunocytochemistry, and Western blotting procedures. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Interval: Maintain cultures at a cell concentration between 0.5 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
mouse IgM |
| Name of Depositor |
SK Malhotra |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Jeffcoat S, et al. A novel intermediate filament-associated protein: further characterization of the G.3.5 antigen. Cytobios 82: 81-99, 1995. PubMed: 7587376
. . Ann. N.Y. Acad. Sci. 436: 492-494, 1984.
Price KA, et al. Localization and characterization of an intermediate filament- associated protein. Cytobios 76: 157-173, 1993. PubMed: 8181306
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