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pCU--1
pCU--1
規(guī)格:
貨期:
編號:B237487
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCU--1
商品貨號 B237487
Designations pCU--1
Species Candida tropicalis (Castellani) Berkhout
Depositors Henkel Research Corp., S Picataggio, Henkel Research Corp.
Vector
Construct size (kb): 16.5
Insert
DNA: genomic
Insert lengths(kb): 5.800000190734863
Gene product: uridine monophosphate synthetase( UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1) [URA3]
Insert Size (kb) 5.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.8, 4.4 (doublet), 2.3, 0.4; EcoRI/HindIII--4.4 (doublet), 2.3, 1.6, 1.4 (doublet), 1.25, 0.75, 0.4; BamHI--uncut; BglI--11.0, 5.0, 2.4; EcoRV--7.2, 3.9, 3.4, 2.2, 1.0, 0.3;
PstI--9.6, 4.3, 2.6, 1.0 (doublet).
This plasmid complements the pyrF mutation in E. coli.
A 6.2 kb EcoRI fragment contains 377 bp of vector sequence and all of the insert except for 50 bp. The gene is in a 2.8 kb EcoRI/SacI fragment.
References

Picataggio S, et al. Site-specific modification of the Candida tropicalis genome. US Patent 5,254,466 dated Oct 19 1993

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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