国产精品乱视频,国产精品va久久久久久久,2021国产精品视频网站,91久久成人免费,乱色熟女少妇一区二区,天天干夜夜操夜夜操,麻豆久久樱花一区二区av,国产高清久久久久

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > pDO6
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
pDO6
pDO6
規(guī)格:
貨期:
編號(hào):B238326
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pDO6
商品貨號(hào) B238326
Designations pDO6
Depositors A Ahmed
Biosafety Level 1
Vector Information
Size (kb): 8.9320001602172850
Vector: pDO6 (cosmid)
Construction: pDO2
Marker(s):ampR,kanR
Construct size (kb): 8.932000160217285
Features: insert detection: lacZ'
marker(s): ampR
marker(s): kanR
other: lambda cos site
replicon: lambda
replicon: oriP
Applications
vector containing primer sites useful for sequencing
vector for generating nested deletions of cloned inserts
vector permitting visual detection of recombinants
Comments
EcoRI--6.6, 2.1; HindIII--5.0, 3.9; SstI--9.0.
Plasmid contains the following restriction sites (approximate kb from nt 1): BamHI--5.91; BglI--5.73, 7.32, 8.53; EcoRI--3.77, 5.88; HindIII--2.25, 5.96; KpnI--5.89; PstI--0.65, 1.72, 5.94, 7.45; SacI--5.88; SmaI--0.93, 4.50, 5.89; XbaI--5.92.
A series of overlapping deletion segments can be generated by partial digestion with a restriction enzyme not found in the vector, followed by complete digestion with lambda terminase.
Resulting fragments are blunted and circularized by ligation, and transformants are selected for either ampicillin or kanamycin resistance.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Cosmid vector containing two compatible origins of replication, for generating bidirectional deletions of large inserts to facilitate sequencing.
AmpR transformants can be sequenced in the clockwise direction using the cosL primer 5'-TCATAAATAGCGAAAACC-3'. KanR transformants can be sequenced in the counterclockwise direction using the cosR primer 5'-ACTTTACGGGTCCTTTCCG-3'.
The order of the major features in the plasmid is: 5' lacZ - Ecl136II/MCS/Sse8387I - 3' lacZ - ren - P - O - ori lambda - cII - cro - kanR - cosR/L - ampR - oriP.
Media ATCC Medium 1065 (see below) plus ampicillin (50.0 mcg/ml) plus kanamycin (20.0 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 30.0°C
References

Ahmed A. Double-origin vectors for isolating bidirectional deletions useful in DNA sequence analysis. Gene 141: 71-73, 1994. PubMed: 8163177

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
济源市| 哈密市| 酒泉市| 尚义县| 蕲春县| 望奎县| 东乡| 金坛市| 甘肃省| 博罗县| 南康市| 师宗县| 夏邑县| 关岭| 七台河市| 焉耆| 武威市| 鹿邑县| 乐平市| 霍山县| 油尖旺区| 新兴县| 云阳县| 琼结县| 邹平县| 长垣县| 纳雍县| 怀化市| 博客| 利川市| 中宁县| 轮台县| 石泉县| 牙克石市| 昭觉县| 荔波县| 读书| 阿坝| 阳泉市| 兴义市| 岱山县|